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Steps to extract DNA and RNA from plant samples includes the following: Disrupt the tissue and homogenize the plant sample, bind nucleic acid with target magnetic beads, wash and elute sample to purify DNA using a magnetic particle processing instrument or manually using microcentrifuge tubes.
Challenges of extracting DNA and RNA from plants can be difficult and require kits specifically designed to mitigate carbohydrates, phenolics, and other compounds found in plant tissues. Cell walls can be difficult to disrupt, and lysates often contain inhibiting compounds such as tannins, phenolics, and complex polysaccharides which may hinder DNA quality, affecting downstream reactions.
For automated high-throughput extraction or manual purification, the MagMAX Plant DNA Isolation Kit is designed to reduce these challenges and effectively extract DNA from a wide variety of plant species. The kit uses MagMAX magnetic bead technology and eliminates the need for phenol/chloroform extraction or alcohol precipitation. The procedures are optimized for the isolation of DNA from 10&#;100 mg plant samples. DNA yields vary between different species and tissues depending on genome size, ploidy, cell number, age of tissue sample, and storage conditions.